PT681. The protective role of erythropoietin on the cognitive power deficit of the brain and histological changes in the hippocampus of diabetic mice.

Objective: HYBID (Hyaluronan Binding Protein Involved in HA Depolymerization, KIAA1199) is a hyaluronan (HA) binding protein, which involves in depolymerization of HA. It is reported that HYBID mRNA is expressed in the lung, heart, skin and brain in murine and human. However, the role of HYBID in the brain remains unclear. In this study, we have made HYBID KO mice and evaluated its function in the central nervous system. Methods: To investigate the role of HYBID in brain, behavioral tests were performed by using HYBID KO mice. In situ hybridization was performed to investigate the localization of HYBID mRNA in mouse brain. Results: HYBID mRNA was expressed in the brain, especially hippocampus and cerebellum in wild-type mice, but not KO. HYBID KO mice showed decreased memory ability in a novel object recognition test. The expression of Hyal1 and Hyal2 mRNAs was not changed in the HYBID KO mouse brain. These results suggest that HYBID plays a key role in memory function in the brain. Conclusion: HYBID may be involved in brain function, such as memory and learning. Policy of Full Disclosure: None. PT681 The protective role of erythropoietin on the cognitive power deficit of the brain and histological changes in the hippocampus of diabetic mice. Amer Al Ansari, Ebrahim Rajab, Manal Othman, Muhammad AlNaisar, Ahmmed Almubarak Arabian Gulf University, College of Medicine and Medical Sciences,